FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-6R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-6R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25382124
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT578610.22795964593034057No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG324820.1279719538049692No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT321790.1267782002798505No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG257940.1016227010789168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA133150.036.8817024
ACTCTAG226750.033.2347265
TCTAGTT151200.032.6622247
GTACTCT157000.030.9667933
CTAGTTG168300.028.8451738
ACCTACT156700.027.316233
CTCTAGT188650.027.2561046
CTAATGC173900.027.13015621
ACACCTA158700.026.6852531
TAGTTGT186300.026.4903649
TGTACTC184050.026.3585222
GTTACCT185000.025.90076314
ATGCTGG182200.025.89436324
ACCTCTA187100.025.5906917
TTGTACT191250.025.147671
TACCTCT192350.025.03778616
TGGACAC188600.024.66310928
GACTATC168450.024.51731955
GGACACC193850.024.03127529
TCTAATG197200.024.03110520
Produced by FastQC (version 0.11.5)