FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-5R-HSFA6A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-5R-HSFA6A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21744232
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT846510.3893032414297272No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT220740.1015165769018653No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG220570.10143839524891014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA166550.045.0365524
GTACTCT177950.042.1511843
TCTAGTT191000.039.7467657
ACTCTAG241600.039.0146755
CTCTAGT218850.035.538096
TGTACTC215650.034.7822232
CTAGTTG216200.034.5156568
TTGTACT217950.034.2233241
TAGTTGT228900.032.830079
ACCTACT200200.032.4471533
CTAATGC224650.032.37447721
ACACCTA202200.032.09173631
GTTACCT234450.031.47016114
ATGCTGG232450.031.13763224
ACCTCTA238300.030.90226617
TACCTCT240800.030.46522716
TGTTACC242850.030.45376613
TGGACAC233600.030.31025928
TCTAATG250000.029.13370320
TAATGCT250050.029.0858222
Produced by FastQC (version 0.11.5)