FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-5G-SVP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-5G-SVP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16186584
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT635290.3924793520362295No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT207270.1280504892199614TruSeq Adapter, Index 19 (97% over 37bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC172890.10681067728681976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC42600.043.2976347
CGTATGC45300.041.48959446
TACTCTA134600.040.983514
TATGCCG46400.039.82713348
GTACTCT142550.038.5505373
ACGTCTG50750.038.06915
ACTCTAG171750.037.3970455
GCACACG52500.036.7340511
TCTAGTT153200.036.576417
CACACGT55350.035.28545812
GTCACGT55100.035.0631229
TCGTATG53450.034.9668645
CTCTAGT164150.034.778846
AGTCACG55800.034.7487128
CTCGTAT50850.033.45094744
AGCACAC59000.032.74630410
CACGTCT59500.032.5300514
ACACGTC59750.032.39393613
CTAGTTG169950.032.3542638
CACGTGA61850.031.63262431
Produced by FastQC (version 0.11.5)