FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-4R-LFY.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-4R-LFY.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17111374
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT858620.5017831998762928No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC257230.15032691121122124No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG186580.1090385845110977No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG175600.10262179997935876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA156000.045.2568244
GTACTCT159250.043.981543
TCTAGTT178350.039.5625847
ACTCTAG230200.037.0707325
TGTACTC200650.035.046412
TTGTACT202250.034.4070241
CTCTAGT215200.034.287436
TAGTTGT226300.031.01039
CTAGTTG228200.030.6293168
ACCTACT209150.030.07131233
ACACCTA212400.029.57822431
GTTACCT236200.029.5030914
TACCTCT239500.028.96445716
CTAATGC238050.028.96419521
TGTTACC241850.028.94418313
ATGCTGG240450.028.45675824
TGGACAC239250.028.13136128
ACCTCTA251850.027.57199517
TCTAATG250950.027.5310920
TAATGCT251300.027.45096422
Produced by FastQC (version 0.11.5)