FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-4G-WRKY33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-4G-WRKY33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15862806
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT819520.516629907722505No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC289680.1826158625403349TruSeq Adapter, Index 2 (97% over 37bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC201430.12698257798777846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC49550.048.66734344
GTATGCC51150.047.00815645
GCACACG54500.045.40419411
TACTCTA160450.044.5909844
TATGCCG54750.043.7254346
GTACTCT163650.043.633233
ACGTCTG58200.042.75768715
ACTCTAG199050.041.4832735
TCGTATG59250.040.2863743
TCTAGTT178950.040.0753977
CACACGT62650.039.77724512
ACCTATA61900.039.40979832
CTCGTAT57900.039.23089642
ACACGTC64650.038.60084513
CACGTCT65150.038.46576314
CTCTAGT191650.038.2086336
CACCTAT65100.037.63389631
AGCACAC67350.037.1053410
CTAGTTG199100.035.5807468
GTCACCT69700.034.94941729
Produced by FastQC (version 0.11.5)