FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-3R-MYB4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-3R-MYB4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27616873
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT720460.2608767473421049No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG644130.23323784702199993No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT583520.21129111901988323No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG506700.1834747909366857No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA478500.1732636421219738No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA459340.16632585448758086No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG447130.161904644309296No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA395370.1431624789671155No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC361810.1310104876826569No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG359060.13001471962448463No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA351360.1272265690616023No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA331910.12018377315925666No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT328460.11893453686809509No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC322960.11694300075175057No Hit
CTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGC317100.1148211095441544No Hit
GCCGGAGGCACGACCCGGCCAATTAAGACCAGGAGCGTATCGCCGACCGA299200.1083395647291422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT127900.048.4939353
TACTCTA134000.046.7305154
TCTAGTT148800.041.8452387
CGTATGC19950.038.24508346
GTATGCC20350.037.83731547
TATGCCG22550.034.30107548
TAGTTGT185550.033.3881579
ACCTACT179000.031.46068833
GGCAGAC121800.031.1234131
TGTTACC197500.030.97818813
ACACCTA181650.030.94397231
GTTACCT197050.030.94207814
CTAATGC195750.030.87829821
TACCTCT200400.030.56411416
CCAGGCA54200.030.416912
ATGCTGG197700.030.21972324
TGGACAC198700.029.89154628
TCTAATG203750.029.73455620
TAATGCT206550.029.31463622
TCGTATG26100.029.09921345
Produced by FastQC (version 0.11.5)