FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-3G-GBF2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-3G-GBF2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16625114
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT2221641.3363156487227696No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC1205800.7252882596774975TruSeq Adapter, Index 3 (97% over 37bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC533080.32064742533494806No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG346560.20845571344653638No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG334630.2012798228030196No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT320250.19263025805417033No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA285300.17160784581687683No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA221240.13307577920969443No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215990.1299179061268392No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT212340.12772243245971127No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA207910.12505778907741624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG161850.061.1761246
GTATGCC161900.061.0275245
CGTATGC162250.060.8095944
GCACACG168800.060.69183311
CACCTAG166350.060.4897731
ACCTAGC169000.059.52020332
CACACGT174550.058.7734512
ACGTCTG176350.058.3309315
CTCGTAT159000.057.95827542
TCGTATG167750.057.8769543
AGCACAC177500.057.7364510
ACACGTC182400.056.24349213
CACGTCT182700.056.20810314
ATGCCGT177650.055.7154847
GTCACCT181500.055.22867229
TCACCTA184200.054.70414730
CCTAGCT185950.054.3582333
GCCGTCT179900.053.8708149
TCCAGTC188900.053.41717525
TAGCTAT181150.053.0550235