Basic Statistics
Measure | Value |
---|---|
Filename | H3KMLBGXC_n01_0801-3G-GBF2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 16625114 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 222164 | 1.3363156487227696 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC | 120580 | 0.7252882596774975 | TruSeq Adapter, Index 3 (97% over 37bp) |
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC | 53308 | 0.32064742533494806 | No Hit |
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG | 34656 | 0.20845571344653638 | No Hit |
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG | 33463 | 0.2012798228030196 | No Hit |
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT | 32025 | 0.19263025805417033 | No Hit |
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA | 28530 | 0.17160784581687683 | No Hit |
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA | 22124 | 0.13307577920969443 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 21599 | 0.1299179061268392 | No Hit |
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT | 21234 | 0.12772243245971127 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 20791 | 0.12505778907741624 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 16185 | 0.0 | 61.17612 | 46 |
GTATGCC | 16190 | 0.0 | 61.02752 | 45 |
CGTATGC | 16225 | 0.0 | 60.80959 | 44 |
GCACACG | 16880 | 0.0 | 60.691833 | 11 |
CACCTAG | 16635 | 0.0 | 60.48977 | 31 |
ACCTAGC | 16900 | 0.0 | 59.520203 | 32 |
CACACGT | 17455 | 0.0 | 58.77345 | 12 |
ACGTCTG | 17635 | 0.0 | 58.33093 | 15 |
CTCGTAT | 15900 | 0.0 | 57.958275 | 42 |
TCGTATG | 16775 | 0.0 | 57.87695 | 43 |
AGCACAC | 17750 | 0.0 | 57.73645 | 10 |
ACACGTC | 18240 | 0.0 | 56.243492 | 13 |
CACGTCT | 18270 | 0.0 | 56.208103 | 14 |
ATGCCGT | 17765 | 0.0 | 55.71548 | 47 |
GTCACCT | 18150 | 0.0 | 55.228672 | 29 |
TCACCTA | 18420 | 0.0 | 54.704147 | 30 |
CCTAGCT | 18595 | 0.0 | 54.35823 | 33 |
GCCGTCT | 17990 | 0.0 | 53.87081 | 49 |
TCCAGTC | 18890 | 0.0 | 53.417175 | 25 |
TAGCTAT | 18115 | 0.0 | 53.05502 | 35 |