FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-2R-DIV2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-2R-DIV2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16191551
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1428380.8821761423596789No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC556890.3439386381205852No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG312590.19305747794019237No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG213870.13208740780917158No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT210010.12970344842195786No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA163510.10098476668479751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA244150.049.7764054
GTACTCT246300.049.2566263
TCTAGTT276450.043.7803737
CGTACGT30850.043.1114634
ACTCTAG303300.042.1232955
TTGTACT291650.041.4541131
GTACGTA32600.040.79719535
TGTACTC297250.040.7902682
CGTATGC31450.040.06300446
CCGTACG33900.039.54243533
CTCTAGT320500.039.3936926
GTATGCC32500.039.09173647
TATGCCG34000.037.77885448
TACGTAA35200.037.0877536
TAGTTGT358900.033.3724679
GCACACG39950.033.20525711
ATGCCGT39400.032.60104449
CTAGTTG369350.032.5040748
CTCGTAT37450.031.40140744
TCGTATG39650.031.24796345