FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-2G-MYB3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-2G-MYB3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23781760
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1003300.4218779434322775No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC263410.11076135660270728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA210150.042.2061044
ACTCTAG256900.040.6295175
GTACTCT221850.039.9170343
GCACACG68200.039.87669811
TCTAGTT242400.036.9206057
CTCTAGT249350.036.006556
ACGTCTG76000.035.9212715
CTAGTTG252050.034.868958
GTATGCC55350.034.71492845
CACACGT80400.033.86950312
ACACGTC80100.033.77744713
TATGCCG55100.033.72907646
CGTATGC54700.033.52783644
ACCTACT247250.031.5102133
AGCACAC87550.031.38304910
ACACCTA254000.030.89343331
CACGTCT88100.030.86897314
GGACACC276700.030.55997529
GTTACCT289250.030.15447414
ACCTCTA289800.029.96376217
Produced by FastQC (version 0.11.5)