FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-1R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-1R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19075871
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT980940.5142307787675855No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC307960.16143954842219263No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG195690.1025850929690183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA182200.046.317074
GTACTCT196050.042.901943
TCTAGTT202250.041.8101047
ACTCTAG252900.038.5865675
TGTACTC235400.035.8194732
CTCTAGT246050.035.5216456
TTGTACT234850.035.4717031
CTAGTTG258900.032.2295278
TAGTTGT262450.031.7669059
GTTACCT268150.030.6350414
ACACCTA247100.030.4956131
TGTTACC273700.030.12908413
ACCTACT249200.030.12603233
CTAATGC271900.030.0308121
TACCTCT276250.029.8756816
ATGCTGG275900.029.39260724
ACCTCTA286550.028.72829217
TCTAATG286150.028.71877320
TGGACAC279900.028.57272328
TTACCTC295200.027.95785115
Produced by FastQC (version 0.11.5)