FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-15R-HSFA6A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-15R-HSFA6A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17333446
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT815860.47068540208334797No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC262380.1513720930044724No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG213100.12294150857250197No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC197310.1138319524000017TruSeq Adapter, Index 2 (97% over 36bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT174390.10060896142636612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA161750.044.274314
GTACTCT170800.041.887413
ACTCTAG210000.041.052125
CGTATGC42600.039.60046844
GTATGCC42250.039.43148845
TCTAGTT184650.039.1039247
GCACACG44700.038.6032211
TGTACTC186250.038.393932
TTGTACT185400.038.0797541
CTCTAGT193050.037.6765566
TATGCCG44850.037.22363746
ACGTCTG50600.034.1698515
CTAGTTG208600.034.1286858
CACACGT53500.032.51541512
ACACGTC53550.032.0925113
TCGTATG53950.030.88007743
TAGTTGT238300.030.0367859
ACCTACT210600.029.4655533
ACCTCTA238950.029.44112817
CTCGTAT52950.029.34809342
Produced by FastQC (version 0.11.5)