FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-15G-SVP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-15G-SVP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20292796
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1379090.6795958526365711No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC762880.3759363667776486TruSeq Adapter, Index 10 (97% over 36bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC244310.12039247819768159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC115400.057.07908244
GTATGCC117000.056.74722745
TATGCCG121200.054.54971746
GCACACG135600.054.41129711
ACGTCTG136250.053.7138215
ACTCGGC137550.052.7983932
CACACGT139650.052.55800212
ACACGTC140000.052.4012213
TCGTATG127450.050.33682643
CGGCAAT137950.050.03206335
CACTCGG147250.049.74829531
AGCACAC148550.049.715310
CACGTCT148250.049.39057514
CTCGTAT120950.048.55671342
TACTCTA241650.048.0451624
TCACTCG152800.047.52903430
TCGGCAA152250.046.91901434
ACTCTAG280750.045.892125
GTACTCT254100.045.759773
GTCACTC159950.045.38253429
Produced by FastQC (version 0.11.5)