FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-14R-LFY.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-14R-LFY.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17498854
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1830351.0459827826439376No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC600990.34344534790678294No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC497620.28437290807729465TruSeq Adapter, Index 9 (97% over 36bp)
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG329890.1885209168554695No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295480.16885677199204016No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT287060.16404502832014028No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG263660.15067272405381518No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA217330.12419670453848006No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA196240.11214448671895885No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG188910.10795564098083223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC78350.053.73896844
GTATGCC80250.053.4697445
TATGCCG80300.052.30321546
GCACACG101300.050.8256911
TACTCTA321150.049.2299844
ACGTCTG106100.048.9213515
GTACTCT323200.048.77683
CTCGTAT83050.048.33775742
CACACGT108650.047.51695312
ACTCTAG360800.047.147485
ACACGTC110250.047.01744513
TCGTATG86400.046.90914543
TTGTACT335400.046.8057631
TGTACTC339450.046.49322
CACGTCT113100.046.0180714
AGCACAC113200.045.6682310
ATGCCGT89700.045.33946247
CTCTAGT354500.044.9150776
TCTAGTT354200.044.447117
TGCCGTC91300.043.5098748