FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-14G-WRKY33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-14G-WRKY33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19996926
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1579780.790011424755985No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC1262110.6311520080636394TruSeq Adapter, Index 10 (97% over 36bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC307480.15376363347046443No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG251000.12551929231522885No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG218000.10901675587537804No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT215480.10775656218360763No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT212270.10615131545718577No Hit
CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGC205080.10255576282074555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC177300.060.64237645
CGTATGC177750.060.54792444
GCACACG189250.059.7924811
TATGCCG179050.059.77600546
ACGTCTG191150.058.92231815
ACACGTC195900.057.53022413
CACACGT197900.057.0553512
CTCGTAT181600.055.94933342
TCGTATG189950.055.7562243
CACGTCT202600.055.6102814
AGCACAC206000.055.03293610
TCACTCG206800.053.8369130
CACTCGA209100.053.26133731
GTCACTC212600.052.31878329
CAGTCAC215550.051.7164127
GCCGTCT199500.051.6835749
AGTCACT216300.051.60181828
CGTCTGA219150.051.4576516
GAGCACA221250.051.398039
TCCAGTC218950.051.1529825