FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-13R-MYB4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-13R-MYB4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12733211
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1817201.4271341297964826No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC561940.44131837601685864No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG294160.23101792627170004No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT260790.20481086820912653No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239660.1882164679435533No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG236810.18597822654474194No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA203360.15970834065342984No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA180860.1420380138207087No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT160090.125726338784459No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT150120.11789642062791546No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA143490.11268956432120697No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA142190.11166861210420528No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT141410.11105604077400429No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG138580.10883350633237758No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA127600.10021038683800969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT306750.050.6733633
TACTCTA309300.050.2671054
TTGTACT316600.048.7897641
ACTCTAG341350.048.264885
TGTACTC322950.047.979732
GCACACG61550.047.88129411
CTCTAGT338250.046.1204456
TCTAGTT343050.045.3099177
ACGTCTG65300.045.18413515
CACACGT65200.045.0936312
CACGTCT68500.043.02309814
AGCACAC68950.042.94552610
ACACGTC69000.042.6100513
TCCAGTC75400.038.75980825
AGTCACC73950.038.62055228
CAGTCAC74750.038.34768727
GAGCACA77600.038.3388449
CTAGTTG410500.037.5759548
CCAGTCA78200.036.96917726
CATGGCA77850.036.91030534
Produced by FastQC (version 0.11.5)