FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-13G-GBF2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-13G-GBF2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16454718
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT3336772.027850006302144No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATGC864170.5251806807020333TruSeq Adapter, Index 6 (97% over 36bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC810680.49267328677404254No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG572620.3479974558056844No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT467480.28410088826803354No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT467400.2840522699933235No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG454370.2761335684999281No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCGCGTATGC406140.24682282613412154TruSeq Adapter, Index 6 (97% over 36bp)
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA389930.23697154822100264No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA325270.1976758276866246No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA270590.1644452369223222No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT267540.1625916651990025No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA254370.15458788172486457No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT246580.14985367722497583No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA239600.1456117327565261No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG221050.13433837030813897No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA201140.12223849718968141No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATGG188810.11474520559999873TruSeq Adapter, Index 6 (97% over 36bp)
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA186370.11326234822134297No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT185830.11293417486705028No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT185400.1126728516404839No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG181870.11052757026890404No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCGCGGATGC169170.1028094191586875TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCCG692750.066.376932
GCACACG708750.066.2781711
CCCGAAT650600.066.02923635
ACGTCTG710600.066.0005815
CACACGT714450.065.7312
ACACGTC714900.065.6435613
CTCCCGA700550.065.2431633
CACGTCT720050.065.19350414
AGCACAC719900.065.1833810
CACTCCC708000.064.98691631
GTCACTC711550.064.89406629
TCCAGTC717900.064.7442125
AGTCACT718900.064.45454428
CAGTCAC717850.064.40742527
CGTCTGA730700.064.1465316
CCAGTCA720300.064.1154626
GAGCACA735150.063.907199
TCCCGAA709750.063.347134
CTGAACT740100.063.1990119
AGAGCAC752800.062.5388228
Produced by FastQC (version 0.11.5)