FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-12R-DIV2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-12R-DIV2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15209700
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1601421.052893876933799No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC496950.32673228268802146No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358210.23551417845190897No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG257490.16929327994635002No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG231390.15213317816919467No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA226360.14882607809489995No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA184310.12117924745392744No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT181450.11929886848524296No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC180080.11839812751073327TruSeq Adapter, Index 7 (97% over 36bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT165130.10856887381079179No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA153000.10059370007297974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT290750.047.7683143
TACTCTA292650.047.5061764
TTGTACT294350.046.626431
TGTACTC301250.045.975552
ACTCTAG318200.044.945645
GTATGCC41800.042.5353245
CTCTAGT333300.041.9757236
GCACACG45050.041.95448311
TCTAGTT332000.041.7686167
CGTATGC43400.040.6446244
TATGCCG44850.039.48665246
ACGTCTG49400.038.3300815
CACACGT51750.036.6585612
ACACGTC52650.035.83165713
AGCACAC54900.035.12853610
CTAGTTG393650.035.049938
ACCAGGC55500.034.17983632
ATGCCGT51700.033.8486847
TAGTTGT413350.033.3964089
TCGTATG51900.033.24618543
Produced by FastQC (version 0.11.5)