FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-12G-MYB3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-12G-MYB3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18786384
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT457950.2437669750602351No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC382260.2034771566470695TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC71300.048.5968145
GCACACG75800.047.2834411
TATGCCG73400.047.11107346
CGTATGC74350.046.65033344
ACGTCTG81550.043.94918415
CACACGT84850.042.52955612
ACACGTC86200.041.74145513
CTCGTAT76400.041.59628742
TCGTATG82350.041.05590443
AGCACAC92500.039.0874110
CACGTCT93000.038.87767414
TACTCTA108500.035.8084374
GTCACTC100850.035.53772429
GAGCACA104250.034.950379
CGTCTGA104650.034.38162616
GCCGTCT98700.034.36123349
ACTCTAG149150.033.7699855
AGTCACT107450.033.55029728
TCCAGTC107800.033.31149725
CAGTCAC108250.033.0113627
Produced by FastQC (version 0.11.5)