FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-11R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-11R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17670170
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT538690.30485841392584223No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG258160.14609933011397175No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT233040.13188328125875415No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG187770.10626383334172788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA121450.039.886854
ACTCTAG183350.037.034975
GTACTCT138750.034.7117583
TCTAGTT141100.034.3800477
TGTACTC147050.032.8477172
CTCTAGT153000.032.3028346
TTGTACT149200.032.047121
CTAGTTG150250.031.8210018
ACACCTA143650.028.94509131
ACCTACT145450.028.41844433
TAGTTGT178850.027.4370049
CTAATGC171600.027.31058921
ACCTCTA173350.027.21667717
GTTACCT176050.026.87901514
GGACACC171250.026.38516429
GACTATC154350.026.37156955
ATGCTGG176800.026.2894324
TGGACAC175300.026.07506428
GACACCT173750.026.04580730
TACCTCT183350.025.73219316
Produced by FastQC (version 0.11.5)