FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-10R-HSFA6A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-10R-HSFA6A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16418415
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1117880.6808696210931444No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC298730.18194813567570317No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG165460.10077708475513622No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG164440.10015583112011725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA206850.046.872184
GTACTCT215350.044.9245873
ACTCTAG250150.043.48825
TTGTACT225100.042.622641
TGTACTC226550.042.5954932
CTCTAGT240650.040.5364766
TCTAGTT239100.040.474867
CTAGTTG269250.035.6050348
TAGTTGT294050.032.7094549
ACACCTA267250.031.8106831
ACCTACT268600.031.78110533
ACCTCTA304100.031.21335217
GTTACCT305000.031.01844214
CTAATGC302550.030.89886521
GGACACC295300.030.6972529
TGTTACC312950.030.38722813
TTACCTC312650.030.3823415
TACCTCT312350.030.31048616
ATGCTGG305700.030.2712624
GACACCT302300.029.99801330
Produced by FastQC (version 0.11.5)