FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-10G-SVP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-10G-SVP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15713242
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1529340.9732810071912593No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC640310.40749706521416773TruSeq Adapter, Index 9 (97% over 36bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC400920.25514785554756936No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG246700.15700133683424464No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG237820.15135005239529817No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT223970.1425358306070765No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA196780.1252319540423294No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191780.12204992451589558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC86800.056.97502544
GTATGCC86850.056.74073445
TATGCCG90200.054.47818846
GCACACG97350.052.3133611
ACGTCTG97750.051.99037615
TCGTATG95200.051.50664543
CACACGT99800.051.1700512
CTCGTAT94600.050.9083942
TACTCTA267250.049.6251874
AGCACAC102400.049.5967310
GTACTCT267950.049.2081533
ACACGTC104650.048.79795513
CACGTCT106700.047.82730514
ACTCTAG298000.047.065035
GAGCACA112800.045.396059
CACTATA112100.045.27160331
TCTAGTT295900.044.900837
CTATAAT113500.044.80541233
TATCTCG107700.044.3585839
AGTCACT114000.044.3331528