H3KCTBGX7_n01_nlp7_7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3KCTBGX7_n01_nlp7_7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7591274
Sequences flagged as poor quality	0
Sequence length	76
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT	396160	5.218623382583741	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	65264	0.8597239409353424	TruSeq Adapter, Index 7 (100% over 50bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG	58338	0.7684876082723401	No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT	43738	0.5761615244028868	No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG	38913	0.5126017055898654	No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC	38227	0.5035650142518898	No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT	35628	0.46932833671923846	No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG	33439	0.4404925971582635	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	27877	0.36722426301566774	No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT	25401	0.3346078668745194	No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG	21893	0.2883969146680781	No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA	20399	0.2687164236200669	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	19919	0.2623933742873726	No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA	19325	0.25456860073816334	No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT	16511	0.21749972402524265	No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA	15116	0.1991233619020997	No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG	14918	0.19651510405236328	No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA	13415	0.17671605582936406	No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA	13313	0.17537240784616653	No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG	13184	0.1736730883380049	No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA	13084	0.1723557863936936	No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA	12808	0.16872003302739436	No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC	12222	0.16100064363372998	No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT	11726	0.15446682598994582	No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC	11573	0.1524513540151495	No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA	10433	0.1374341118500004	No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT	9821	0.1293722239508151	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9698	0.12775194255931216	No Hit
TTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCT	9561	0.12594723889560566	No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT	8100	0.10670145748921724	No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA	8001	0.10539732856434901	No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA	7930	0.10446204418388798	No Hit
ATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCC	7766	0.1023016689952174	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	8060	63.35568	44
CTCGTAT	7580	63.257763	42
TATGCCG	8170	62.50308	46
TCGTATG	8075	62.501156	43
GTATGCC	8340	61.31256	45
ATGCCGT	8585	58.992836	47
GCCGTCT	8620	58.428093	49
CCGTCTT	8820	57.063133	50
TTGTACT	57245	56.924526	1
CAGTCAC	9190	56.89807	27
AGTCACC	9190	56.669563	28
CATCTCG	8410	55.516525	39
AAAAAGG	8450	55.21194	69
TGAAAAA	9320	55.052574	62
ACGTCTG	9755	55.038097	15
CAGATCA	9375	54.953957	34
CACGTCT	9955	53.791725	14
CCAGTCA	9735	53.60486	26
ACCAGAT	9790	53.124603	32
TCTCGTA	8850	53.07241	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content