FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7924545
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT4810016.069761733954442No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG702540.8865367033690893No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC609620.7692807599679224TruSeq Adapter, Index 6 (100% over 50bp)
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT532890.6724550116126541No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG470840.5941539861279101No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC450140.5680326125979472No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT443000.5590226315832644No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG404110.5099472588016094No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT294640.37180683559750116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG273350.34494093982682916No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG264020.33316739320680244No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT261080.32945740102428595No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA255440.32234027316394825No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT211990.267510626793084No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA202100.2550304149954351No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA196630.24812781049258978No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG172650.2178673980651255No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA171430.21632787749959145No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA169800.21427097707186973No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG158580.2001124354773681No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC158140.1995571985520935No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA156480.1974624410612849No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA152480.19241483264969786No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC146550.18493175317952007No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146430.18478032492717247No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT144280.18206723540594444No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA133510.16847654975774634No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT123300.15559252928717043No Hit
TTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCT109310.13793851886764477No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA105860.13358495661265093No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT102050.1287771096006143No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT99650.12574854455366208No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA98110.12380521531520107No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCAT95980.12111736383603096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95240.12018355627988736No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC91080.11493404353183684No Hit
ATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCC89180.112536429536333No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG84430.1065423945475734No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC82030.10351382950062116No Hit
AATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTC80570.1016714524303919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT74750.061.1954242
CGTATGC77000.060.72538444
TATGCCG77700.060.26839446
TCGTATG77600.060.07545543
GTATGCC79450.058.80874345
AGTCACG80600.058.40426328
TTGTACT692850.057.288061
GTCACGC82650.056.91329229
ATGCCGT82600.056.52402547
GCCGTCT83400.055.93985749
CCAATAT83750.055.91503535
ACGCCAA84300.055.4671932
CGCCAAT85200.054.88126433
GCCAATA85650.054.71586234
CACGCCA85800.054.70144331
TATCTCG83600.054.67568239
CAGTCAC87150.054.2958427
CCGTCTT86500.053.81369450
CAATATC86200.053.47315236
ACGTCTG90950.053.41297515
Produced by FastQC (version 0.11.2)