FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6574990
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT3331425.066806185256556No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC500450.7611418420408244TruSeq Adapter, Index 3 (100% over 50bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG486350.7396969425048555No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT374940.5702518178734872No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG344480.5239247512163516No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC326960.4972783228567648No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT312030.4745710639864091No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG298290.45367369380029476No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216520.32930848564028237No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT213450.32463927701791184No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG200060.3042742270330449No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT177350.26973425054638867No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA168960.2569737748650568No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA148350.22562771958588532No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT146750.22319425580875407No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA139130.21160488457016663No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA121280.18445655430654648No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG120830.1837721426192283No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG119040.18104970501856277No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA115950.1763500780989781No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC115580.17578733960051648No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG113520.17265425498746007No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA110790.16850215741772992No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA109590.1666770595848815No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT103370.1572169691512839No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC101800.15482913281997387No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94340.14348310795909955No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA88130.134038226674109No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCAT87600.1332321417979343No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT82620.12565798579161336No Hit
TTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCT75780.1152549281443774No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA74230.11289751011028153No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT71940.10941461507926248No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71190.10827392893373222No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA70490.1072092885312373No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT68810.10465415156524953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC63050.062.9489544
TATGCCG64950.060.89148346
CTCGTAT61800.060.7675642
TCGTATG66050.060.08980643
GTATGCC66600.059.4880145
ATGCCGT68600.057.7030847
TTGTACT512150.056.507631
GCCGTCT69900.056.47970649
CCGTCTT71650.055.00253750
CATCTCG66300.054.9010139
AGTCACT73400.054.59715328
TTAGGCA72900.054.5391134
TCACTTA74100.054.22308330
TCTCGTA67700.054.02417841
CAGTCAC74900.053.55047627
GCATCTC68300.053.2421338
CACTTAG75400.053.24178331
GTCACTT75300.053.12657529
AAAAAGG70250.052.36208369
ACTTAGG76900.052.24837532
Produced by FastQC (version 0.11.2)