FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12721198
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT277750.21833635479928856TruSeq Adapter, Index 19 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT245720.1931579085554678No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG244480.1921831575925475No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA180400.14181054331518148No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG158940.1249410629407702No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG149660.11764615250859235No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGG146580.11522499689101608No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT139200.10942365648266776No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG138270.10869259326047752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45300.052.6148348
CGTATGC49550.048.45512446
GTATGCC50000.047.9492247
TCGTATG56700.042.22136745
CTCGTAT52050.041.35379444
ACGTGAT62550.038.9437532
AGTCACG62500.038.8633728
GTCACGT62450.038.8384429
GCCGTCT65200.036.8242651
TCACGTG66150.036.66592430
CACGTGA66450.036.44772331
CAGTCAC71400.034.1662427
ACGTCTG72750.033.868615
GCACACG75500.032.5425211
GGCAGAC40050.032.5161171
TCTCGTA65950.032.2664743
TACTCTA68500.031.325094
CACGTCT79150.031.13025714
CACACGT79050.031.03669212
CCTACTA63250.030.8213934