FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7854805
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT2930203.7304554346034053No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC616130.7843988488574828TruSeq Adapter, Index 2 (97% over 37bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG451220.5744509252616711No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT346050.44055835886441486No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG303280.38610761183759496No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC296600.37760326322550336No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT288360.36711286912915086No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG286070.36419745620674226No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG277160.3528540810370213No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG216490.2756147351843871No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT172500.21961079874038883No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT162520.20690520006543764No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA147530.18782133993141778No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA141260.17983896481198453No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA124480.1584762447953832No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT114630.1459361499107871No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA110490.14066549074101775No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG107990.13748272554188168No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCAT107000.1362223505230238No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA101260.1289147216258074No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA98890.12589746021702639No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC97420.12402599427993438No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT96850.12330032381453136No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG90910.11573807370138406No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA89820.11435038807456072No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC86730.11041649028842855No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA84490.10756473267000263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC80250.060.3163744
TATGCCG80300.059.8429646
CTCGTAT79850.058.8214442
GTATGCC83050.058.07211345
TCGTATG84750.056.8246543
ATGCCGT89950.053.50139647
GCCGTCT90150.053.34387649
TTGTACT479300.051.8116651
CCGTCTT94800.050.8011750
TCTCGTA91100.050.6739241
CATCTCG91300.050.2952139
GTACTCT523150.047.6924133
TACATCT96850.047.59372737
TGTACTC526500.047.4022522
TGCCGTC102050.047.32925448
TACTCTA533200.046.9772954
AAAAGGG97750.046.79687570
CTATACA103550.046.44085734
ACTCTAG539850.046.3272975
TCTAGTT539700.046.135887
Produced by FastQC (version 0.11.2)