FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12004188
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG246610.2054366359473877No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC239870.19982192881351077TruSeq Adapter, Index 3 (97% over 37bp)
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA216690.18051200131154227No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG199820.1664585726248206No Hit
GGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGG178780.14893135628998813No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT163480.13618580448756717No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT160680.13385328520346398No Hit
CGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGAT134430.11198591691499665No Hit
CGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTC132330.11023652745191928No Hit
GCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA123320.10273081361271584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36650.048.79792446
CGTATGC39200.045.8916144
GTATGCC41300.043.7274445
CACCTAG44750.040.3564431
TCGTATG46250.038.82072443
CTCGTAT45450.037.9639242
ACCTAGC47850.037.7417632
TAGCTAT51800.034.2558235
TCACCTA52950.034.23891430
GCCGTCT55500.032.60284849
CCTAGCT57550.031.74531233
GTCACCT58250.031.00344729
GCTATCT55650.030.62822237
ACGTCTG62150.029.67788115
TCTCGTA58050.029.24147841
AGTCACC63350.028.728528
GCACACG64400.028.47772411
TACTCTA51950.028.3010884
TATCTCG60400.028.16171839
CACACGT65300.027.97814612