H3KCTBGX7_n01_nlp7_33.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3KCTBGX7_n01_nlp7_33.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6481039
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT	196793	3.0364421507107116	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC	39399	0.6079117869835376	TruSeq Adapter, Index 7 (97% over 37bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC	31977	0.4933931118143249	No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG	31279	0.4826232337129896	No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT	25610	0.39515269079541104	No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG	25522	0.3937948838141539	No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT	23537	0.3631670786119324	No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG	20586	0.3176342558654561	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	20454	0.3155975453935704	No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG	17580	0.2712528037556941	No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCAT	15262	0.23548693349939726	No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA	13051	0.2013720330953108	No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT	12758	0.1968511530327159	No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA	9935	0.1532933222589773	No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT	9741	0.1502999750502967	No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG	9596	0.1480626794561798	No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG	7599	0.1172497187565142	No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA	7537	0.11629308201971937	No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA	7530	0.11618507464621027	No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC	7500	0.11572218590259988	No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA	7475	0.11533644528292455	No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA	7458	0.11507414166154531	No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT	7188	0.11090814296905172	No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG	7061	0.10894858062110103	No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA	6818	0.10519918179785678	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	4990	58.07454	46
CGTATGC	5105	56.90341	44
CTCGTAT	5035	56.443317	42
GTATGCC	5155	56.215702	45
TCGTATG	5210	55.555077	43
ATGCCGT	5700	50.59548	47
CGGAATA	5845	49.938732	34
GCCGTCT	5825	49.14923	49
TCTCGTA	5935	47.884094	41
TATCTCG	5975	47.56353	39
CAGTCAC	6375	46.226524	27
AGTCACC	6410	45.75536	28
GAATATC	6330	45.55955	36
CCGTCTT	6345	45.176395	50
ACGTCTG	6995	44.881504	15
CCAGTCA	6735	43.547752	26
AATATCT	6800	43.182625	37
TCCAGTC	7070	43.117947	25
CACGTCT	7310	42.94782	14
TGCCGTC	6800	42.668262	48

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content