FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12912790
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT345240.2673628239907874No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC281600.21807835487141045TruSeq Adapter, Index 2 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258120.1998948329524448No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA218180.16896425946677673No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG207580.16075534412005463No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT169110.13096317681926214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46800.047.8629146
CGTATGC47600.047.2051844
GTATGCC52050.043.10231445
TCGTATG56750.039.71747243
CTCGTAT53150.039.4443842
TACTCTA83500.034.4580464
GTCACCC65750.034.38705429
GCCGTCT65800.033.67049449
TCTAGTT88150.032.5983737
GTACTCT88800.031.6522483
ACGTCTG75700.030.88413615
TCTCGTA68200.030.6890541
AGTCACC74900.030.326328
ACTCTAG118750.030.2134725
CACCCAA76050.029.63772831
GCACACG79150.029.44997411
CTAGTTG95750.029.4253798
TCACCCA76700.029.29529830
CAGTCAC78150.029.06513227
CCTACTA90700.028.9025534
Produced by FastQC (version 0.11.2)