FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10179210
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT846570.8316657186559664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG234740.2306072868130238No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG179310.17615315923337863No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA177260.17413925049193404No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC159890.15707505788759638TruSeq Adapter, Index 12 (97% over 37bp)
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG159190.15638738173198116No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT157760.15498255758551008No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA146930.1443432250636346No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC143390.14086554850523764No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT141610.13911688628095892No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG138860.13641530138389915No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA129200.1269253704364091No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT124510.12231794019378714No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG122960.12079522870635344No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG122810.12064786953015016No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA117420.11535276313191299No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGA116680.11462579119597689No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA115220.11319149521426515No Hit
CCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAA112980.11099093151629646No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT106030.10416328968554533No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG103700.10187431048185468No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT103010.10119645827131969No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT102400.10059719762142642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26100.054.7114346
CGTATGC25400.054.56571644
GTATGCC27200.052.62750645
TCGTATG25950.051.52126743
CTCGTAT25850.049.2834842
GTACTCT140750.048.7947433
TACTCTA143600.047.8267944
TCTAGTT146750.046.8907627
AGTCACC40950.039.57177428
CAGTCAC41050.039.4751827
CATCTCG31250.039.42389739
GGCATCT32600.038.75757237
GCCGTCT36050.038.34885449
TTGTACT180200.038.0167961
TGTTACC177950.037.8412513
CATGGCA42550.037.42627734
GGCAGAC35350.037.233851
GTTACCT180750.037.061614
TACCTCT181250.036.97903416
GCATCTC33650.036.6120938
Produced by FastQC (version 0.11.2)