Basic Statistics
Measure | Value |
---|---|
Filename | H3KCTBGX7_n01_nlp7_29.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 10345783 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 105729 | 1.0219526158629078 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC | 30741 | 0.29713555755035653 | TruSeq Adapter, Index 7 (97% over 36bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 25266 | 0.24421544507554432 | No Hit |
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG | 14981 | 0.1448029598146414 | No Hit |
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT | 13602 | 0.1314738575127663 | No Hit |
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG | 12986 | 0.12551974074847694 | No Hit |
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC | 12768 | 0.12341260202345244 | No Hit |
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT | 11964 | 0.11564131975317865 | No Hit |
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG | 11751 | 0.11358250989799419 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 5140 | 0.0 | 51.20523 | 46 |
CGTATGC | 5380 | 0.0 | 49.18096 | 44 |
GTATGCC | 5605 | 0.0 | 46.956932 | 45 |
CTCGTAT | 5185 | 0.0 | 46.913033 | 42 |
TCGTATG | 5770 | 0.0 | 45.31086 | 43 |
TACTCTA | 19550 | 0.0 | 44.655655 | 4 |
GTACTCT | 19990 | 0.0 | 43.69004 | 3 |
TCTAGTT | 20065 | 0.0 | 43.539757 | 7 |
TTGTACT | 20250 | 0.0 | 42.78459 | 1 |
ACTCTAG | 20960 | 0.0 | 42.219856 | 5 |
CCAGGCG | 6425 | 0.0 | 41.781086 | 33 |
CTCTAGT | 21090 | 0.0 | 41.594254 | 6 |
TGTACTC | 20985 | 0.0 | 41.567837 | 2 |
CAGGCGA | 6305 | 0.0 | 41.466084 | 34 |
CTAGTTG | 22020 | 0.0 | 39.13336 | 8 |
ACCAGGC | 6890 | 0.0 | 38.80893 | 32 |
ATGCCGT | 6905 | 0.0 | 38.01574 | 47 |
TAGTTGT | 22920 | 0.0 | 37.963207 | 9 |
GCCGTCT | 6920 | 0.0 | 37.781605 | 49 |
ACGTCTG | 7440 | 0.0 | 36.739697 | 15 |