FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10345783
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1057291.0219526158629078No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC307410.29713555755035653TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG252660.24421544507554432No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG149810.1448029598146414No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT136020.1314738575127663No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG129860.12551974074847694No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC127680.12341260202345244No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT119640.11564131975317865No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG117510.11358250989799419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG51400.051.2052346
CGTATGC53800.049.1809644
GTATGCC56050.046.95693245
CTCGTAT51850.046.91303342
TCGTATG57700.045.3108643
TACTCTA195500.044.6556554
GTACTCT199900.043.690043
TCTAGTT200650.043.5397577
TTGTACT202500.042.784591
ACTCTAG209600.042.2198565
CCAGGCG64250.041.78108633
CTCTAGT210900.041.5942546
TGTACTC209850.041.5678372
CAGGCGA63050.041.46608434
CTAGTTG220200.039.133368
ACCAGGC68900.038.8089332
ATGCCGT69050.038.0157447
TAGTTGT229200.037.9632079
GCCGTCT69200.037.78160549
ACGTCTG74400.036.73969715