FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10100911
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1626861.6106072016672555No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC427410.42314005142704453TruSeq Adapter, Index 7 (97% over 35bp)
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG250310.24780933125734894No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG232550.23022675875473014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG232210.22989015545231511No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT201630.19961565842922485No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG196700.19473491054420733No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG178090.17631082978555102No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC163940.16230219234680912No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT162070.1604508741835266No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC158350.15676803805122133No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT156720.15515432221905529No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG152870.1513427848240619No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA145310.14385831139389307No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA141950.1405318787582625No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC140320.13891816292609646No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT139000.13761135010495587No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT130940.12963187181829441No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA130350.12904776608763308No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG130060.12876066327086735No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAA120000.11880116555823528No Hit
GGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAG111750.11063358542610661No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT109640.10854466493170764No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG109410.10831696269772104No Hit
GGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCT103370.10233730403128986No Hit
CCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAA103080.10205020121452411No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG101600.10058498683930588No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA101580.10056518664504617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG58150.061.99385546
CGTATGC59450.060.87371444
GTATGCC59900.060.41639745
CTCGTAT56400.059.94584742
TCGTATG60650.059.5538743
ACTCAAT65250.053.58481635
GCCGTCT68050.053.33466749
CCACTCA69750.051.98431433
CAGTCAC71400.051.6653327
GTACTCT257650.051.5854763
AGTCACC71900.051.1113328
TACTCTA263350.050.442624
ACCACTC72500.050.2538732
TCTAGTT265500.049.870747
CACTCAA72500.049.626334
CTCAATC66950.049.29670736
CCGTCTT73450.048.9370350
CCAGTCA75800.048.2507326
TTGTACT275300.048.1805731
ACGTCTG77700.048.15262215
Produced by FastQC (version 0.11.2)