H3KCTBGX7_n01_nlp7_26.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3KCTBGX7_n01_nlp7_26.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9913715
Sequences flagged as poor quality	0
Sequence length	76
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT	104400	1.0530865573601822	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC	28358	0.28604816660555604	TruSeq Adapter, Index 2 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	21700	0.21888868098386932	No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC	17987	0.18143551635285057	No Hit
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG	17205	0.17354745420863923	No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA	16668	0.16813071588198775	No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA	16390	0.1653265198767566	No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG	16010	0.16149344620054137	No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG	15815	0.15952647418248356	No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT	15230	0.15362555812831014	No Hit
CTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTG	14983	0.15113406023877024	No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAA	14034	0.1415614630842222	No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT	13090	0.1320393011096244	No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT	12873	0.1298504142997857	No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG	12648	0.12758083120202668	No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT	12396	0.12503889813253657	No Hit
CCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAA	12147	0.12252722617101662	No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC	11657	0.11758457853589699	No Hit
GGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAG	10612	0.10704362592630512	No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG	10561	0.10652918709081309	No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA	10504	0.1059542260393808	No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATG	10414	0.10504639280027718	No Hit
CGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACT	10395	0.10485473911646644	No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT	10270	0.10359385961771143	No Hit
CCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAG	10135	0.102232109759056	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	3935	58.880596	46
CGTATGC	4015	57.79456	44
GTATGCC	4190	55.380703	45
CTCGTAT	3725	55.15321	42
TCGTATG	4210	55.11761	43
GTACTCT	17205	50.028004	3
TACTCTA	17645	48.919357	4
TCTAGTT	17765	48.249577	7
GCCGTCT	4835	48.21044	49
AACTAAT	4910	47.046333	35
TAATCTC	4535	44.994	38
CAGTCAC	5295	44.749054	27
ACGTCTG	5350	44.68176	15
AGTCACC	5325	44.43122	28
TTGTACT	20035	42.825314	1
CACGTCT	5635	42.29768	14
CTAATCT	4865	42.229755	37
CCGTCTT	5565	41.446095	50
TCTCGTA	4945	41.33382	41
CCAGTCA	5705	41.103436	26

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content