FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8732672
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1043121.194502667682927No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG485560.5560268380628518No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC337840.3868689903846154TruSeq Adapter, Index 22 (97% over 37bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC257490.2948582060565197No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA229560.2628748680816135No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG226330.25917611471036583No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197680.22636828681988744No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG149530.17123052371599437No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC143960.16485217811913697No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC117530.1345865274683396No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA115850.13266271766533771No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG111390.12755546068832083No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT108990.12480716096974671No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG99740.11421475580440901No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA97650.11182144479948407No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC95550.1094166825457317No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG94070.10772189771927768No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT93140.10665693157833021No Hit
GGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTG91440.10471021927767354No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG90260.10335897191604128No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG87740.10047325721153845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG47550.057.19140246
CGTATGC47850.057.1254144
GTATGCC49050.055.51378345
CTCGTAT47700.054.1502842
TCGTATG51300.052.94281843
ATGCCGT52850.051.78714847
GCCGTCT54750.049.03108249
TTGTACT178750.048.3719061
GGTAGCA56750.048.22820334
GTAGCAT56050.047.83143235
GTACTCT182900.047.7120973
TACTCTA184600.047.3675234
AGTCACG58400.047.3453128
TCTAGTT186850.046.7173847
CATCTCG54900.045.77383439
TGTACTC192150.045.3606072
TCTCGTA56700.044.69106341
ACTCTAG217800.043.490295
CTCTAGT205700.042.6623656
TGCCGTC63800.042.40519348