FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8718132
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT828520.9503411969444829TruSeq Adapter, Index 22 (97% over 40bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG659380.7563317462961102No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT469160.5381428039859915No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC380050.4359305410838009No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG366200.42004411036676204No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA338960.3887988848987375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269880.3095617272140408No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC216550.24839036619312485No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC173020.19845994531856137No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG170760.19586764687664743No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA155370.17821478270803884No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG138980.15941488383061875No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG138840.15925429897138516No Hit
GGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTG137350.15754521725525605No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG136800.15691434816540975No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA136000.15599672039836057No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG132010.1514200519102028No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC129160.1481510029900901No Hit
GGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGC121600.13947942059147533No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT117540.13482245967370074No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC115530.1325169199089897No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA111740.1281696583625942No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG105820.12137921288643026No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG99760.11442818255103272No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG98160.11259292701693437No Hit
TGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATC96590.11079208252410035No Hit
CGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTCGTAGTGGCCG95760.10984004371578683No Hit
GGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGAT94170.10801625852877657No Hit
CTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAA93760.10754597429816386No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT92320.10589424431747534No Hit
GGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTC92320.10589424431747534No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT92000.10552719321065568No Hit
GTGATGTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGG91870.1053780786985102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG109100.061.4332648
CGTATGC110400.060.74156646
GTATGCC111000.060.3820547
TCGTATG113650.058.727445
ATGCCGT114200.058.47521249
CTCGTAT106700.058.41966244
TACGTAA115650.057.8933836
CGTACGT117150.057.3310334
GTACGTA117200.057.21730835
GCCGTCT117150.056.6442251
CCGTACG120250.055.96947533
ACCGTAC123250.054.63584532
TCTCGTA114150.053.99368743
CTGCTTG126200.052.52881659
CAGTCAC129800.051.79790527
CCGTCTT128450.051.7973552
AGTCACC130900.051.5230628
TGCCGTC130700.050.9592250
ACGTAAT132500.049.89713737
GTAATCT124000.049.6764539
Produced by FastQC (version 0.11.2)