FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15095843
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG977570.6475756272769927No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG525750.3482746872764906No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC508140.336609224142037No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT496530.3289183651419798No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA496210.328706386254812No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320170.2120915009516196No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC299920.1986772119980315No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT269520.17853921771708942TruSeq Adapter, Index 21 (97% over 40bp)
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC257250.17041115226224862No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG249140.16503881234058937No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA237510.15733470466008426No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG205840.13635541917069488No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG203450.13477220185716027No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA201460.13345395815258543No Hit
GGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTG191810.12706146983643113No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG189530.12555112026536047No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG182820.12110618797506043No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC181490.1202251507252692No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT172370.11418375244098657No Hit
GGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGC167200.11075896854518162No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC162480.1076322799594564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46150.047.47513648
GTATGCC48000.045.7182847
CGTATGC49100.044.55103746
ATGCCGT54800.039.66200349
TCGTATG57350.037.7760545
CTCGTAT54350.036.96337544
TACTCTA111350.036.84224
TCTAGTT113600.036.3251767
GTACTCT117050.034.8386463
AGTCACG67100.034.1125828
GTCACGT68750.033.3956929
CGTTTCG69000.033.32552333
GCCGTCT65000.033.11504751
ACTCTAG154300.033.097795
CTAGTTG128250.031.7930498
CTCTAGT131800.031.6571586
TTGTACT126650.031.4545941
TAGTTGT130800.031.2267469
CCTACTA119950.031.22081634
TGCCGTC71700.030.50875350
Produced by FastQC (version 0.11.2)