FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12050051
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT607760.5043630105797893No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG456140.37853781697687416No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC260280.21599908581299782No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA251160.2084306531150781No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245230.2035095121174176No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG238950.1982979159175343No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT231140.1918166155479342TruSeq Adapter, Index 20 (97% over 44bp)
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTC163090.13534382551575924No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAA145520.12076297436417488No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC142330.11811568266391569No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT138260.11473810359806777No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATG125370.10404105343620539No Hit
GGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCG122060.10129417709518408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC37350.051.81958446
TATGCCG37950.050.44779248
GTATGCC38200.050.3006747
ATGCCGT40800.047.0096549
CTCGTAT39750.046.92988644
TCGTATG41450.046.69388245
GCCTTAT41650.045.5458837
GTACTCT115650.045.098423
TACTCTA116350.045.0376854
TCTAGTT118600.044.237527
TTGTACT123250.041.808541
GGCCTTA46650.041.63921736
AGTCACG47650.040.91226228
GTCACGT47650.040.7653629
TCACGTG48150.040.5599430
TGTACTC132750.039.2629242
CACGTGG50900.038.43734731
TATCTCG47600.038.0145841
GCCGTCT50900.037.40655551
TAGTTGT139600.037.3561489
Produced by FastQC (version 0.11.2)