FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9788138
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG590180.6029543106155635No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT568150.5804474763228716No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC321820.32878572002151996No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG290010.2962871998739699No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA274570.28051300461844736No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT204370.20879354173388237TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201180.20553449491619347No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC188100.19217138131889844No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC144020.14713727983810607No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA140320.1433571941874951No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG135550.13848394863251826No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA114180.1166513998883138No Hit
GGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGAT111370.11378057808339032No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG109380.11174750499022387No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG109330.1116964227517021No Hit
GGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTG109320.11168620630399775No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG106520.10882560094677865No Hit
CGGCCCTCGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG106270.1085701897541698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33800.048.7716848
CGTATGC33900.048.2145946
GTATGCC35350.046.73242647
CTCGTAT35600.043.94594244
ATGCCGT37850.043.4603549
TACTCTA112300.043.2016334
TCGTATG38200.043.15377445
TCTAGTT114850.042.5121777
GTACTCT114000.042.3117643
TTGTACT116600.040.9206851
ACTCTAG142450.039.5869525
TGTACTC124450.038.7309382
CTAGTTG130400.037.146478
CTCTAGT132050.036.9261366
TAGTTGT130650.036.8342939
GCCGTCT43750.036.7194351
CCTACTA127450.034.9857234
ACCTACT128000.034.698533
TGTTACC136800.034.58957313
GTTACCT137400.034.1585114
Produced by FastQC (version 0.11.2)