FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6366009
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT3715165.835932685611975No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC629530.988892726981693TruSeq Adapter, Index 12 (100% over 50bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG574750.9028419532551714No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT434570.6826411963916482No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC420000.6597540154278764No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG403540.6338979413946791No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT400140.6285570755555011No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG368280.5785100209566151No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG279490.4390348804093743No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG273910.43026957706154667No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT254560.39987376706504807No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA226710.35612579247060444No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA200860.3155195036639125No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT198240.31140389528195767No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG168250.2642943168946195No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT168210.2642314831788645No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA149510.23485672106338526No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA142950.22455199167955936No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG136170.2139016768590808No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA134900.2119067063838584No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA129720.20376974019358124No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA128560.2019475624366852No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT124090.19492589470105998No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC119260.18733872352363937No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115040.1807097665114831No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC114720.1802070967854428No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA112880.17731674586071117No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC105990.16649363832190622No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG103510.16259794794509402No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102170.1604930184673003No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCAT100360.15764979282938493No Hit
TTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCT96230.1511622116776775No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT95330.14974845307318918No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA92580.14542863511503049No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92150.14475317267066384No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA82040.1288719510135785No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT79370.12467780048692988No Hit
ATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCC78250.12291845644578887No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT76250.11977677065803707No Hit
AATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTC72960.11460869753718538No Hit
TGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAA71890.11292789564073817No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC70940.11143559489155608No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT66960.10518364017393No Hit
AGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAG64470.10127224136817901No Hit
GAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGAT63790.10020406820034343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC81000.061.87415744
CTCGTAT76300.061.60361542
TCGTATG81450.061.44685743
TATGCCG82300.060.72670446
GTATGCC82900.060.45605545
TGTAATC79600.058.25872836
GCCGTCT85150.058.0374349
ATGCCGT85950.058.026647
TTGTAAT87000.056.60215835
TAATCTC81900.056.58035338
CAGTCAC89150.056.2966527
CTTGTAA88850.056.28977634
AGTCACC90450.055.52665328
CCGTCTT89750.055.21879650
AAAAAGG84350.054.9771469
TGAAAAA91800.054.78547362
AAAAGGG85900.054.066670
TTGTACT570350.053.6849331
TCTCGTA87650.053.18802341
GTAATCT87800.052.73838837
Produced by FastQC (version 0.11.2)