FastQCFastQC Report
Thu 12 Jul 2018
H3KCTBGX7_n01_nlp7_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KCTBGX7_n01_nlp7_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8517195
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT5042525.9203998499506No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG736860.8651439822617658No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC687250.8068971063830288TruSeq Adapter, Index 10 (100% over 50bp)
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT557120.6541120638895787No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC512060.6012073223637594No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG506930.5951842126427773No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT459580.5395907925085666No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG415010.48726135775921536No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG301000.3534027341161028No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT300980.3533792522068592No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG275400.32334589028430133No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA264260.310266466835619No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT261900.3074956015448748No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA212280.24923698471151592No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT208790.2451393915485086No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA197730.23215389573680068No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA189060.2219744880797023No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG181770.21341533216041197No Hit
CCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGG179830.21113758696378326No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA178930.2100809010478215No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC160750.18873584554539377No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158040.18555404684288665No Hit
TTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTA157480.184896553384066No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA156510.18375768078575164No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT148080.1738600560395764No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC148050.173824833175711No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA147270.1729090387152108No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT131300.1541587341842003No Hit
CTGTTACTACCCTACACTCATACTTGGATCACACTATGTACTAGGAGCAT130730.15348949977075788No Hit
TTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCT116340.13659426606999137No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA115640.13577239924646553No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112490.13207399854059934No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT105220.12353832453055262No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA103290.1212723202885457No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT102370.12019215246334035No Hit
ATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCC94340.11076416590203698No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC91280.10717143378776697No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG90500.10625563932726678No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC86870.10199367279955432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC82500.061.55638544
CTCGTAT81050.060.67125742
TCGTATG84000.060.49883343
TATGCCG84800.060.0102846
GTATGCC86750.058.66134645
TTGTACT736550.057.025731
ATGCCGT89550.056.7099147
CTTATCT86050.056.49546437
TTATCTC87550.055.52817538
GCCGTCT92100.054.83575449
AGTCACT95600.054.18305628
CAGTCAC96350.053.94323327
CCGTCTT94000.053.6901450
TATCTCG91000.053.34606639
ACGTCTG104150.052.6259315
GCTTATC95300.051.5995236
AAAAAGG91400.051.57936569
GTACTCT819250.051.5631833
CACGTCT106250.051.4866714
TCTCGTA94500.051.4446641
Produced by FastQC (version 0.11.2)