FastQCFastQC Report
Sat 30 Jun 2018
H3K23BGX7_n01_del_ltp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3K23BGX7_n01_del_ltp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135481166
Sequences flagged as poor quality0
Sequence length151
%GC38

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAAAAAAT4824100.35607163286445287No Hit
CGATCAAGATCGGCAGAGATCCAGTATCGTTAAGTAAGGTACCAAAAAAT2035840.1502673810764221No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAAGAAAT1775780.13107209307602208No Hit
CGATCAAGATCGGCAGAGATCCAGTATCGTTAATTAAGGTACCAAAAAAT1691030.12481661104097673No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAATAAAT1687020.12452062894114743No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAAGAAAC1505670.11113500455111229No Hit
CGATCAAGATCGGCAGAGATCCAGTATCGTTAAGTAAGGTACCAAAAAAG1452290.107194973506502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG28405650.0144.908313
CGCGCGG28372250.0144.883544
CGATCGC35355100.0144.875536
GCGCGGT28451500.0144.822455
CGCGGTG28451600.0144.795766
ATCGCGC28372500.0144.75372
GCGGTGC28434600.0144.660117
CGGTGCA35060700.0144.654838
ATCGCAG41198200.0144.642688
CGATCAA28484950.0144.62341
ACGATCG35423050.0144.621465
TCGCAGA41260300.0144.569699
ATACACG36220500.0144.56921
TACACGA36274350.0144.561362
GATCGCA35393900.0144.555857
GATCGGC33981850.0144.529148
GATCGCG28468800.0144.39911
ACACGAT35647050.0144.358893
ATCGGCA34075800.0144.110669
CACGATC35730250.0144.004044