FastQCFastQC Report
Sat 30 Jun 2018
H3K23BGX7_n01_del_etp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3K23BGX7_n01_del_etp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135347544
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCCTTACCAGCAGAGATCCAGAAAGGTTAATTAAGGTACCAAAAAA20135561.487693045985378No Hit
TCGATCCTTACCAGCAGAGATCCAGATAGGTTAATTAAGGTACCAAAAAA8756020.646928621032089No Hit
TCGATCCTTACCAGCAGAGATCCAGAAAGGTTACTTAAGGTACCAAAAAA6113640.45169936737086264No Hit
TCGATCGTTACCAGCAGAGATCCAGAAAGGTTAATTAAGGTACCAAAAAA5751250.42492459264720756No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAAAAAAT5637400.4165129143385122No Hit
TCGATCCTTACCAGCAGAGATCCAGAAAGGTTAATTAAGGTACCATAAAA5133040.37924884695358785No Hit
TCGATCCTTACCAGCAGAGATCCAGTAAGGTTAATTAAGGTACCAAAAAA4863910.3593644817079208No Hit
TCGATCCTTACCAGCAGAGATCCAGTTAGGTTAATTAAGGTACCAAAAAA4450950.3288533998075355No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAAAAAAG4183510.30909389829785167No Hit
TCGATCGTTACCAGCAGAGATCCAGATAGGTTAATTAAGGTACCAAAAAA3607700.2665508285839306No Hit
TCGATCCTTACCAGCAGAGATCCAGATAGGTTACTTAAGGTACCAAAAAA3343420.24702480009537522No Hit
TCGATCCTTACCAGCAGAGATCCAGAAAGGTTAATTAAGGTACCAGAAAA2967420.21924446593578384No Hit
TCGATCCTTACCAGCAGAGATCCAGAAAGGTGACTTAAGGTACCAAAAAA2878750.21269318340937168No Hit
CGATCAAGATCGGCAGAGATCCAGTATCGTTAAGTAAGGTACCAAAAAAT2633050.1945399171779578No Hit
TCGATCCTTACCAGCAGAGATCCAGAAAGGTTACTTAAGGTACCAGAAAA2628340.19419192416228845No Hit
TCGATCGTTACCAGCAGAGATCCAGTTAGGTTAATTAAGGTACCAAAAAA2577680.1904489674374882No Hit
TCGATCCTTACCAGCAGAGATCCAGAAAGGTTACTTAAGGTACCATAAAA2574540.19021697209370864No Hit
TCGATCCTTACCAGCAGAGATCCAGATAGGTTAATTAAGGTACCATAAAA2446010.18072067861090998No Hit
TCGATCGTTACCAGCAGAGATCCAGAAAGGTTACTTAAGGTACCAAAAAA2394860.17694151879106135No Hit
CGATCAAGATCGGCAGAGATCCAGTAAGGTTAATTAAGGTACCAAAAAAT2300970.17000456247658252No Hit
TCGATCGTTACCAGCAGAGATCCAGTAAGGTTAATTAAGGTACCAAAAAA2268180.1675819104630373No Hit
CGATCAAGATCGGCAGAGATCCAGTAAGGTTAAGTAAGGTACCAAAAAAT2160170.15960171394022488No Hit
CGATCAAGATCGGCAGAGATCCAGTTACGTTAATTAAGGTACCAAAAAAT1904440.14070739251833042No Hit
TCGATCCTTACCAGCAGAGATCCAGAAAGGTTAATTAAGGTACCACAAAA1896560.14012518764285814No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAATAAAT1857470.13723706726440488No Hit
TCGATCCTTACCAGCAGAGATCCAGAAAGGTGACTTAAGGTACCAGAAAA1854220.1369969446952063No Hit
TCGATCCTTACCAGCAGAGATCCAGTAAGGTTACTTAAGGTACCAAAAAA1736600.1283067242062405No Hit
CGATCATGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAAAAAAT1704080.12590402083690563No Hit
CGATCAAGATCGGCAGAGATCCAGTAAGGTTAATTAAGGTACCAAAAAAG1694310.12518217545196092No Hit
TCGATCCTTACCAGCAGAGATCCAGTTAGGTTACTTAAGGTACCAAAAAA1651570.12202437895733077No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAAGAAAT1623750.11996892976499078No Hit
CGATCAAGATCGGCAGAGATCCAGTATCGTTAATTAAGGTACCAAAAAAG1615700.11937416463205272No Hit
CGATCAAGATCGGCAGAGATCCAGTATCGTTAAGTAAGGTACCAAAAAAC1591950.11761942278021684No Hit
TCGATCGTTACCAGCAGAGATCCAGATAGGTTACTTAAGGTACCAAAAAA1529810.11302827925713968No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAATAAAG1437600.10621544783996967No Hit
CGATCAAGATCGGCAGAGATCCAGTATCGTTAATTAAGGTACCAAAAAAC1426480.10539385923397325No Hit
CGATCAAGATCGGCAGAGATCCAGTAACGTTAAGTAAGGTACCAAGAAAC1408000.1040284853635763No Hit
TCGATCCTTACCAGCAGAGATCCAGATAGGTTACTTAAGGTACCAGAAAA1382970.1021791721614099No Hit
TCGATCCTTACCAGCAGAGATCCAGAAAGGTGACTTAAGGTACCATAAAA1364030.10077981171198792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCC30400050.0144.717931
CGATCAA31037450.0144.630251
GATCGGC37711100.0144.606418
TCGATCG8697450.0144.5841
CGATCCT27697500.0144.562852
CGATCGT8700000.0144.40422
CGATACC30243300.0144.35143
TCGATAC30295950.0144.340652
ATCGATA30246650.0144.293931
ATCGGCA37815800.0144.199
GATCAAG31239300.0143.917022
CCTTACC27733850.0143.608666
CGTTACC8762450.0143.60036
GATCGTT8753100.0143.550493
TGATCGG6622800.0143.26927
TACCAGC38549900.0143.240229
ATCAAGA31448000.0143.154193
TCAAGAT31484350.0142.902394
ATGATCG6639700.0142.879786
CTTACCA27871950.0142.865197
Produced by FastQC (version 0.11.2)