FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37351120
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2381590.6376221114654661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT124350.030.5855183
TACTCTA131800.030.1037834
TCTAGTT139950.027.7227757
ACTCTAG213900.023.9636925
GTTACCT167200.022.22253614
CTAATGC174100.021.20646921
TGTTACC177700.021.18513
CTCTAGT197550.021.0233976
TACCTCT182450.020.65147216
TGTACTC184050.020.4961932
TGGACAC170850.020.44640228
ACCTACT128150.020.16517433
TTGTACT184550.019.1610431
CTAGTTG198000.019.1192538
TCAGGAC124350.019.02562160
GACTATC137650.018.27789155
ACCTCTA220700.017.34184617
GGACTAT160600.015.59916454
CTAGCAG60300.015.02765758
CCTACTA174600.015.02088334
Produced by FastQC (version 0.11.5)