FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-9R-EV.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-9R-EV.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21873327
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT291050.13306160512298837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA98250.031.6677514
TCTAGTT108200.028.9806357
GTACTCT108650.028.4753783
ACCTACT90950.025.748233
ACACCTA98050.024.02443331
CTAATGC130300.023.07403421
GTTACCT133900.022.68680214
TGGACAC131700.022.27091228
TAGTTGT140500.022.144589
ATGCTGG137600.022.00352924
TACCTCT140800.021.62418616
TGTTACC142700.021.5575313
GACTATC104950.021.37566655
CTGTAGG108900.021.11540249
TCTAATG144150.021.0496820
TAATGCT146350.020.71137422
TATCAGG111400.020.32666258
CCTACTA116850.020.1610834
TCAGGAC108250.020.14216460
CTATCTA120950.019.56559638
Produced by FastQC (version 0.11.5)