FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-8R-FBH3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-8R-FBH3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17189027
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC881950.513088960765493No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT860400.5005518927860197No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA318840.1854904294466464No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG266650.15512803604299416No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG251410.14626191465055002No Hit
CAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGCT222550.12947213358848061No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA181530.10560807194031402No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT180990.10529391803270771No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA174600.1015764301260333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA257850.038.044634
GTACTCT256850.038.0153963
TGTACTC283100.034.428772
ACTCTAG314350.034.379835
TTGTACT283350.033.86361
TCTAGTT296850.033.05657
CTCTAGT326200.030.8457326
ATCTAGG284200.023.68052570
TAGTTGT436350.022.3283429
CTAGTTG436850.022.2947778
AATCTAG329450.020.8104369
TGTTACC466950.020.78244613
CCAGCAT152650.020.6621421
GTTACCT472300.020.55450214
ACCTACT345150.020.4639126
TACCTCT478100.020.2539116
ACACCTA352250.020.17078824
GCATTAG208300.019.7752533
ACTACCC151850.019.7287776
ACCTCTA494900.019.61592117
Produced by FastQC (version 0.11.5)