FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-8G-ABF1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-8G-ABF1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29050955
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT524960.1807031817026325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA151400.033.8427774
GTACTCT161050.031.4670223
TCTAGTT175400.029.5096427
ACCTACT149250.026.12772833
ACTCTAG267300.025.4535335
ACACCTA159950.024.57465431
CTAATGC206750.023.76783221
GTTACCT211650.023.46360414
GACTATC165250.023.02098555
ATGCTGG216700.022.51565424
TACCTCT221100.022.50808116
CTCTAGT250150.022.3297926
TGGACAC216850.022.01574528
TCTAATG226200.021.89324620
TGTTACC230000.021.74370413
CAGGACT172250.021.74034161
TCAGGAC174500.021.64052860
TATCAGG177950.021.43720258
CTAGTTG237650.021.4267968
TAATGCT234700.020.9672622
Produced by FastQC (version 0.11.5)