FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-7R-HSFA6A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-7R-HSFA6A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28723955
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG348470.1213168590467434No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG291190.10137531548145093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA87650.023.3984684
TCTAGTT103750.020.5084237
ACTCTAG219650.020.3470845
GTACTCT102050.019.7880523
CTAGCAG106900.019.5115688
ACCTACT75400.019.4995633
CTAATGC112000.017.34354621
ACACCTA85750.016.98116331
CTGACTC124050.016.814812
GTTACCT119400.016.73593914
ATGCTGG116900.016.58743924
GTGCGCA170950.015.86603168
TAGTTGT136800.015.4772449
TGGACAC123250.015.44900828
CTGTAGG92950.015.43827849
TACCTCT127900.015.37738816
GACTATC90400.015.29181655
TGTTACC132500.015.23979113
TGCGCAT178800.015.20859769
GACTGTC101650.014.9080984
Produced by FastQC (version 0.11.5)