FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-7G-ZAT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-7G-ZAT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25027929
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1296490.5180172918022902No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC448590.179235764972803No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA326970.13064205192527115No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA320320.12798502025477218No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT306040.12227939435180593No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT282880.11302573217304557No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA281650.1125342812024119No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG272700.10895827617219146No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA262670.10495075321653662No Hit
CTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTA250750.1001880738913715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT285550.043.3881153
TACTCTA289400.043.0044864
TCTAGTT326500.038.094347
TTGTACT375300.032.7418481
TGTACTC384000.032.328122
ACCTACT310300.030.94391433
ACACCTA314600.030.50758731
TAGTTGT410750.030.2130329
TCAGGAC312950.029.1993360
GTTACCT422400.029.0067314
TGTTACC423050.028.99525313
TACCTCT426450.028.73113816
CACCTAC337800.028.61109532
CTAATGC427700.028.42873621
TGGACAC416300.028.4092728
ATGCTGG431750.027.9519124
CAGGACT327600.027.9256161
TCTAATG438200.027.8579120
ACTCTAG490850.027.7655
TAATGCT439550.027.63057522