FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-6R-CCA1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-6R-CCA1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24099416
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT307340.12753006130936947No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA251550.10438012273824394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA100900.031.2874014
GTACTCT107350.029.4400733
TCTAGTT114300.028.2610287
ACCTACT94100.024.17949733
ACACCTA100150.022.89154631
GTTACCT137400.022.59309414
CTAATGC135800.022.39688521
TAGTTGT146250.021.9919099
ATGCTGG139300.021.65881224
GACTATC103950.021.48025755
TACCTCT144800.021.46215216
TGGACAC138700.021.17183728
TGTTACC149500.021.06881513
TCTAATG149550.020.52352520
TCAGGAC107900.020.272560
TAATGCT151300.020.17203322
AATGCTG154000.019.95477523
CTGTAGG114200.019.95132449
ACTCTAG249600.019.8131085
TATCAGG112150.019.75383258
Produced by FastQC (version 0.11.5)