FastQCFastQC Report
Tue 27 Aug 2019
H3JVYBGXC_n01_0730-6G-SVP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3JVYBGXC_n01_0730-6G-SVP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29057300
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA93850.021.5176754
GTACTCT106700.018.6966063
TCTAGTT114650.018.4674427
ACTCTAG218300.016.786015
ACCTACT88850.015.956079533
CGCTATG76250.015.79059929
CTAATGC126900.015.2245621
CGCGGGG24450.015.0337011
GACTGTC115900.015.0081384
ACACCTA95450.014.92486531
GTTACCT133950.014.42200114
GTCGCTA83400.014.1853627
ATGCTGG137400.014.06161924
GGCTGCC84800.013.95212743
TGGACAC134950.013.87589128
CAGGGGA75750.013.8641771
GACTATC99050.013.85039755
CTAGCAG86000.013.79538
TACCTCT143650.013.56998916
CCTACTA106700.013.35251434
Produced by FastQC (version 0.11.5)